PRP -A2M Procedures

Partial list of medical journals, blogs, and books Dr. Pourcho has been featured in for his expertise on regenerative medicine. Namely, platelet rich plasma (PRP) and Alpha-2-Macroglobulin (A2M) Procedures.

regenerative medicine - PRP - A2M

1.     Tortorella MD, Arner EC, Hills R, et al. Alpha2-macroglobulin is a novel substrate for ADAMTS-4 and ADAMTS-5 and represents an endogenous inhibitor of these enzymes. J Biol Chem. Apr 23 2004;279(17):17554-61. doi:10.1074/jbc.M313041200

2.     Wan R, Hu J, Zhou Q, Wang J, Liu P, Wei Y. Application of co-expressed genes to articular cartilage: new hope for the treatment of osteoarthritis (review). Mol Med Rep. Jul 2012;6(1):16-8. doi:10.3892/mmr.2012.859

3.     Kaneko M, Tomita T, Nakase T, et al. Expression of proteinases and inflammatory cytokines in subchondral bone regions in the destructive joint of rheumatoid arthritis. Rheumatology (Oxford). Mar 2001;40(3):247-55. doi:10.1093/rheumatology/40.3.247

4.     Maiotti M, Monteleone G, Tarantino U, Fasciglione GF, Marini S, Coletta M. Correlation between osteoarthritic cartilage damage and levels of proteinases and proteinase inhibitors in synovial fluid from the knee joint. Arthroscopy. Jul-Aug 2000;16(5):522-6. doi:10.1053/jars.2000.4632

5.     Kapila S, Lee C, Richards DW. Characterization and identification of proteinases and proteinase inhibitors synthesized by temporomandibular joint disc cells. J Dent Res. Jun 1995;74(6):1328-36. doi:10.1177/00220345950740061301

6.     Rengel Y, Ospelt C, Gay S. Proteinases in the joint: clinical relevance of proteinases in joint destruction. Arthritis Res Ther. 2007;9(5):221. doi:10.1186/ar2304

7.     Wilkinson DJ, Arques MDC, Huesa C, Rowan AD. Serine proteinases in the turnover of the cartilage extracellular matrix in the joint: implications for therapeutics. Br J Pharmacol. Jan 2019;176(1):38-51. doi:10.1111/bph.14173

8.     Wang S, Wei X, Zhou J, et al. Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression. Arthritis Rheumatol. 2014;66(7):1843-1853

9.     Brighton CT, Wang W, Clark CC. The effect of electrical fields on gene and protein expression in human osteoarthritic cartilage explants. J Bone Joint Surg Am. Apr 2008;90(4):833-48. doi:10.2106/JBJS.F.01437

10.  Burrage PS, Mix KS, Brinckerhoff CE. Matrix metalloproteinases: role in arthritis. Front Biosci. Jan 1 2006;11:529-43. doi:10.2741/1817

11.  Tchetverikov I, Lohmander LS, Verzijl N, et al. MMP protein and activity levels in synovial fluid from patients with joint injury, inflammatory arthritis, and osteoarthritis. Ann Rheum Dis. May 2005;64(5):694-8. doi:10.1136/ard.2004.022434

12.  Liu X, Liu L, Zhang H, et al. MiR-146b accelerates osteoarthritis progression by targeting alpha-2-macroglobulin. Aging (Albany NY). Aug 17 2019;11(16):6014-6028. doi:10.18632/aging.102160

13.  Woolley DE, Roberts DR, Evanson JM. Small molecular weight beta 1 serum protein which specifically inhibits human collagenases. Nature. May 27 1976;261(5558):325-7. doi:10.1038/261325a0

14.  Salvesen G, Enghild JJ. alpha-Macroglobulins: detection and characterization. Methods Enzymol. 1993;223:121-41. doi:10.1016/0076-6879(93)23041-k

15.     Beekman B, Drijfhout JW, Ronday HK, TeKoppele JM. Fluorogenic MMP activity assay for plasma including MMPs complexed to alpha 2-macroglobulin. Ann N Y Acad Sci. Jun 30 1999;878:150-8. doi:10.1111/j.1749-6632.1999.tb07681.x

16.     Luan Y, Kong L, Howell DR, et al. Inhibition of ADAMTS-7 and ADAMTS-12 degradation of cartilage oligomeric matrix protein by alpha-2-macroglobulin. Osteoarthritis Cartilage. Nov 2008;16(11):1413-20. doi:10.1016/j.joca.2008.03.017

17.     Anitua E SM, Orive G, Andía I. The potential impact of the preparation rich in growth factors (PRGF) in different medical fields. Biomaterials. 2007;28(31):4551-4560. 

18.     Boswell SG CB, Sundman EA, Karas V, Fortier LA. Platelet-rich plasma: a milieu of bioactive factors. Artroscopy. 2012;(3):429-439. 

19.     Foster TE PB, Mandelbaum BR, Gerhardt MB, Rodeo SA. Platelet-rich plasma: from basic science to clinical applications. Am J Sports Med. 2009;37(11):2259-2272. 

20.     Dohan Ehrenfest DM RL, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol. 2009;(3):158-167. 

21.     Anitua W AM, Nurden AT, Zalduendo MM, de la Fuente M, Azofra J, Andia I. Platelet-released growth factors enhance the secretion of hyaluronic acid and indue hepatocyte growth factor production by synovial fibroblasts from arthritic patients. Rheumatology (Oxford). 2007;46(12):1769-1772. 

22.     van Drumpt RA, van der Weegen W, King W, Toler K, Macenski MM. Safety and Treatment Effectiveness of a Single Autologous Protein Solution Injection in Patients with Knee Osteoarthritis. Biores Open Access. 2016;5(1):261-8. doi:10.1089/biores.2016.0014

23.     Orlowsky EW, Kraus VB. The role of innate immunity in osteoarthritis: when our first line of defense goes on the offensive. J Rheumatol. Mar 2015;42(3):363-71. doi:10.3899/jrheum.140382

24.  Montesano P, Cuellar J, Scuderi G. njection of an Autologous Alpha-2-Macroglobulin (A2M) Concentrate Alleviates Back Pain in FAC-Positive Patients. Ortho & Rheum Open Access J. 2017;4(2)